This 2nd Edition of the Outbreak Investigation and Response Guide includes 4 important sections such as: Part 1 is about basic knowledge and procedures in epidemic investigation, which those steps can be used in all epidemics and all incidents, Part 2 is knowledge about diseases and specific symptoms and includes both instructions for collecting, maintaining and sending samples for analysis, symptoms and interaction measures for diseases and symptoms that may cause an epidemic; Part 3 is guidelines for collecting specific samples of cases in people and environments; And the 4th section contains the appendix which includes the data collection form, risk assessment, the form used in the investigation and various documents necessary to carry out each stage of the investigation.